Results and Discussion
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Off-line reaction monitoring
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Investigations of enzymatic reactions
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Matrix solution
Contents
Results and Discussion
Subsections
Off-line reaction monitoring
Reaction assay
Data acquisition and processing
Data processing with
kassandra
Requirements
Quantification
Determination of kinetic parameters
5',3'-GpC transesterification
5',3'-ApC transesterification
Hydrolysis of the cyclic 2',3'-phosphodiester
Conclusions
On-line reaction monitoring using
nano
ES
Development of a
nano
ES ion source
Construction and Design
Enzymatic hydrolysis of 5',3'-GpC by RNase T1
Multiple substrate monitoring
Conclusions
Cleavage pattern analysis
Substrate design
Analysis of cleavage pattern
RNase T1 wild type
RNase T1
RV
Conclusions
Gunter Kuhnle 2001-06-04
