List of Figures

1. Stereoimage of RNase T1
2. Reaction mechanism of RNase T1
3. Binding of the guanosine base moiety in the binding site of RNase T1
4. Relative concentrations of the compounds of enzymatic 5',3'-GpC cleavage
5. Calibration curve for 5',3'-GpC quantification
6. Time-concentration data for 5',3'-GpC transesterification
7. Concentration-rate curve for 5',3'-GpC transesterification by RNase T1 wild type
8. Concentration-rate curve for 5',3'-ApC transesterification by RNase T1 RV
9. Concentration-rate curve for 2',3'-cGMP ester hydrolyis by RNase T1 wild type
10. Construction of the NANOES capillary holder
11. Mass Spectrum of the reaction compounds of enzymatic 5',3'-GpC transesterification
12. Spectrum of cytidine and cytidine$\cdot$2 complex
13. On-line monitoring of 5',3'-GpC hydrolysis by RNase T1
14. Mass spectrum of reaction assay after 200 minutes
15. Mass spectrum of reaction assay after 45 minutes with 1,000 nM RNase T1
16. RNase T1 RV on-line monitoring with multiple substrates
17. RNase T1 RV on-line monitoring with multiple substrates with high enzyme concentration
18. Cleavage positions of R/8
19. R/8 and possible RNase T1 hydrolysis products
20. Mass spectrum of R/8
21. R/8 and possible RNase T1 RV hydrolysis products