Contents

• Contents
• List of Figures
• List of Tables
• Introduction
  • Mass spectrometry
    • Development of mass spectrometry
    • Mass spectrometric techniques
  • Ribonuclease T1: a model enzyme
    • Reaction mechanism
    • Specificity of RNase T1
    • Protein design and specificity alteration
    • Kinetic investigations of RNase T1
  • Enzymatic reactions
    • Kinetic investigations of enzymatic reactions
  • Conceptual formulation
• Experimental
  • Materials
    • Chemicals and Solvents
    • Instrumentation
    • Software
  • Methods
    • Sample preparation
    • Mass spectrometry
• Results and Discussion
  • Off-line reaction monitoring
    • Reaction assay
    • Data acquisition and processing
    • Data processing with kassandra
    • Determination of kinetic parameters
    • Hydrolysis of the cyclic 2',3'-phosphodiester
    • Conclusions
  • On-line reaction monitoring using nanoES
    • Development of a nanoES ion source
    • Enzymatic hydrolysis of 5',3'-GpC by RNase T1
    • Multiple substrate monitoring
    • Conclusions
  • Cleavage pattern analysis
    • Substrate design
    • Analysis of cleavage pattern
    • Conclusions
• Summary
• Bibliography
• Abbreviations
  • General abbreviations
  • Nucleotides
  • Amino acids
• Publications resulting from this work
  • Publications
  • Presentations at Conferences
• Curriculum vitae
• Acknowledgments
• Selbständigkeitserklärung
• About this document ...